Protein-binding properties of 22-oxa-1.ALPHA.,25-dihydroxyvitamin D3, a synthetic analogue of 1.ALPHA.,25-dyhydroxyvitamin D3.

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Cytoplasmic and nuclear binding components for 1alpha25-dihydroxyvitamin D3 in chick parathyroid glands.

Specific binding of 1 alpha,25-dihydroxyvitamin D3 [1alpha,25-(OH)2D3] to macromolecular components in the cytoplasm and nucleus is demonstrated in parathyroid glands of vitamin-D-deficient chicks. The interaction of 1alpha,25-(OH)2D3 with the cytoplasmic binding component is of high affinity (Kd = 3.2 X 10(-10) M) and high specificity [1alpha,25-(OH)2D3 greater than 25-hydroxyvitamin D3 greate...

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22-Oxa-1alpha,25-dihydroxyvitamin D3 inhibits metastasis and angiogenesis in lung cancer.

1alpha,25-Dihydroxyvitamin D(3) (1alpha,25-D(3)) has potent antiproliferative and anti-invasive properties in vitro in cancer cells. However, its calcemic effect in vivo limits its therapeutic applications. Here, we report the efficacy of 22-oxa-1alpha,25-dihydroxyvitamin D(3) (22-oxa-1alpha,25-D(3)), a low calcemic analog of vitamin D, against the development of metastatic lung carcinoma after...

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Phosphorylation of the 1,25-dihydroxyvitamin D3 receptor. A primary event in 1,25-dihydroxyvitamin D3 action.

1,25-Dihydroxyvitamin D3 is believed to mediate the regulation of calcium metabolism by a steroid hormone receptor mechanism. We now demonstrate that phosphorylation of the 1,25-dihydroxyvitamin D3 receptor occurs rapidly in embryonic chick duodenal organ culture in response to 1,25-dihydroxyvitamin D3. Phosphorylation of the receptor is strongly induced within 1 h by 1,25-dihydroxyvitamin D3 a...

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Decreased 1,25-Dihydroxyvitamin D3 Receptor

The resistance of parathyroid cells to 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) in uremic hyperparathyroidism is thought to be caused, in part, by a 1,25(OH)2D3 receptor (VDR) deficiency in the parathyroids. However, results of biochemical studies addressing VDR numbers in the parathyroids are controversial. Several studies have found VDR content to be decreased in the parathyroids of uremic pati...

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Biochemical characterization and purification of a binding protein for 24,25-dihydroxyvitamin D3 from chick intestine.

An earlier study revealed that 24R,25-dihydroxyvitamin D(3) (24R,25(OH)(2)D(3)) inhibits the rapid actions of 1,25(OH)(2)D(3) on stimulation of calcium transport in perfused duodena, as well as activation of protein kinases C and A. In the present work, a specific binding protein (24,25-BP) has been identified and partially characterized. Percoll-gradient resolution of differential centrifugati...

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ژورنال

عنوان ژورنال: Journal of Nutritional Science and Vitaminology

سال: 1989

ISSN: 0301-4800,1881-7742

DOI: 10.3177/jnsv.35.529